Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?
Date
2022-05-17
Journal Title
Journal ISSN
Volume Title
Publisher
ASM Journals
Abstract
Azithromycin is a clinically important drug for treating invasive salmonellosis despite poor activity in laboratory assays for MIC. Addition of the main buffer in blood, bicarbonate, has been proposed for more physiologically relevant and more predictive testing conditions. However, we show here that bicarbonate-triggered lowering of azithromycin MIC is entirely due to alkalization of insufficiently buffered media. In addition, bicarbonate is unlikely to be altering efflux pump activity.
Description
Keywords
Antimicrobial Susceptibility Testing