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dc.contributor"European Union (EU)" and "Horizon 2020"
dc.contributor.authorRekker, Kadri
dc.contributor.authorSaare, Merli
dc.contributor.authorEriste, Elo
dc.contributor.authorTasa, Tõnis
dc.contributor.authorKukuškina, Viktorija
dc.contributor.authorRoost, Anne Mari
dc.contributor.authorAnderson, Kristi
dc.contributor.authorSamuel, Kadri
dc.contributor.authorKarro, Helle
dc.contributor.authorSalumets, Andres
dc.contributor.authorPeters, Maire
dc.date.accessioned2019-02-26T13:08:52Z
dc.date.available2019-02-26T13:08:52Z
dc.date.issued2017
dc.identifier.urihttps://doi.org/10.1530/REP-17-0092
dc.identifier.urihttp://hdl.handle.net/10062/63413
dc.description.abstractThe aetiology of endometriosis is still unclear and to find mechanisms behind the disease development, it is important to study each cell type from endometrium and ectopic lesions independently. The objective of this study was to uncover complete mRNA profiles in uncultured stromal cells from paired samples of endometriomas and eutopic endometrium. High-throughput mRNA sequencing revealed over 1300 dysregulated genes in stromal cells from ectopic lesions, including several novel genes in the context of endometriosis. Functional annotation analysis of differentially expressed genes highlighted pathways related to cell adhesion, extracellular matrix–receptor interaction and complement and coagulation cascade. Most importantly, we found a simultaneous upregulation of complement system components and inhibitors, indicating major imbalances in complement regulation in ectopic stromal cells. We also performed in vitro experiments to evaluate the effect of endometriosis patients’ peritoneal fluid (PF) on complement system gene expression levels, but no significant impact of PF on C3, CD55 and CFH levels was observed. In conclusion, the use of isolated stromal cells enables to determine gene expression levels without the background interference of other cell types. In the future, a new standard design studying all cell types from endometriotic lesions separately should be applied to reveal novel mechanisms behind endometriosis pathogenesis.et
dc.language.isoenget
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/692065///WIDENLIFEet
dc.relation.ispartofseriesReproduction;154(1):93-100
dc.rightsinfo:eu-repo/semantics/openAccesset
dc.titleHigh-throughput mRNA sequencing of stromal cells from endometriomas and endometriumet
dc.typeinfo:eu-repo/semantics/articleet


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