Characterization of plasma membrane proton pump AHA1 mutants
Date
2020
Authors
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Publisher
Tartu Ülikool
Abstract
The gas exchange of plants is regulated by microscopic pores, stomata. Each stoma consists
of two specialized guard cells and a pore that can be closed or open according to prevailing
environmental conditions. For the stomatal opening, various ions and water need to
accumulate in the guard cells. The entry of these osmolytes is driven by energy produced by
the plasma membrane proton pump AHA1, which is considered as the main proton pump in
stomata of Arabidopsis thaliana. The role of AHA1 in guard cells has been studied for a long
time, although its regulation and functioning are not completely understood. The aim of this
Master's thesis was to study newly-identified mutations in AHA1, which could clarify some of
these aspects. Experiments with the AHA1 backcrossed mutant lines and generated
transgenic plants corresponding to some of these mutations bring structural insight for the
AHA1 regulation and highlight its importance for CO2-induced stomatal movements
Description
Keywords
stomata, guard cells, gas exchange, proton pump