Show simple item record

dc.contributor.advisorVahter, Tanel
dc.contributor.advisorHerodes, Koit
dc.contributor.authorAleihela Yamannalage, Ayesh Piyara Wipulasena
dc.contributor.otherTartu Ülikool. Loodus- ja täppisteaduste valdkondet
dc.date.accessioned2021-09-30T10:09:38Z
dc.date.available2021-09-30T10:09:38Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/10062/74374
dc.description.abstractThe Bligh and Dyer method of extraction and separation of phospholipid fatty acids (PLFAs) and neutral lipid fatty acids (NLFAs) has been extensively used in soil microbial community studies. A high throughput method of soil fatty acid analysis was developed which involves the extraction of fatty acids, separation of the fatty acids to PLFAs and NLFAs using solid-phase extraction technique, and subjecting the fatty acids to mild alkaline methanolysis prior to analysis using a GC-FID. Where possible stages were modified to be carried on a 96 well plate multi-tier unit, increasing the sample throughput and decreasing the overall consumption of solvents, cost of analysis, and time. The novel high throughput method showed comparable biomass and community characteristics to that of the standard method and thus, could be proposed as a novel method of fatty acid analysis.et
dc.language.isoenget
dc.publisherTartu Ülikoolet
dc.rightsopenAccesset
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPLFAet
dc.subjectNLFAet
dc.subjectGC-FIDet
dc.subjecthigh throughput methodet
dc.subjectfatty acid analysiset
dc.subjectsoilet
dc.titleDevelopment of a high-throughput method for soil fatty acid derivatization and analysiset
dc.typeThesiset


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

openAccess
Except where otherwise noted, this item's license is described as openAccess