Effect of soil sample preparation on fatty acid biomarker content
Date
2021
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Tartu Ülikool
Abstract
Different techniques for the pre-treatments were applied at various time frames to evaluate their impact on fatty acid biomarkers content for the soil sampled from Suurmetsa, Põlva County, Estonia, one from the arable field the other from grassland. The fatty acids were extracted
by the PLFA method and analysed by using GC-FID. The study included eight bacterial biomarkers (i15:0, a15:0, i16:0, a17:0, 17:0, 10Me17:0 ,10Me18:0, cy19:0),total fungal biomass 18:2ꙍ6,9 and AM fungal biomass by group specific NLFA (16:1ꙍ5). Three pre-treatment methods ( Freezing and Lyophilization, Silica gel, and Hot Air Oven) on four different time frame (0, 8, 24, 48) was carried out. Comparing different pre-treatment methods showed both freezing and lyophilization, and the silica gel method is adequate to identify fatty acid biomarkers content. Still, soil pre-treatment should be done as early as possible to get better results. However, for studying general ecological patterns, whichever approaches, or treatment methods can be chosen.
Description
Keywords
Phospholipid fatty acids, Neutral lipid fatty acids, Fatty acid biomarkers, Pre-treatment methods