Mutso, Margit, juhendajaSerova, EvgeniiaTartu Ülikool. Loodus- ja täppisteaduste valdkondTartu Ülikool. Tehnoloogiainstituut2023-06-202023-06-202023https://hdl.handle.net/10062/90782N6AMT1, also known as HEMK2, is an evolutionally conserved protein which belongs to the human TRMT112-methyltransferase network. It is involved in the methylation of various substrates. Previous studies have shown that this significant methyltransferase is engaged in the majority of vital processes like regulating of cell proliferation, cell cycle factors, etc. Therefore, in the present research, it was studied how the depletion of N6AMT1 affected the viability of U2OS cells. Using CRISPR/Cas9 genome editing system we obtained an N6AMT1 knock-out cell line that showed stunted growth, delayed cell cycle progression, and alterations in morphology. Moreover, it was noticed that N6AMT1 reduction had an impact on cytoskeleton remodelling, namely the β-tubulin level. In conclusion, human N6AMT1 was considered as a potential target responsible for cell cycle and cell cytoskeleton remodelling.engembargoedAccessAttribution-NonCommercial-NoDerivatives 4.0 InternationalmethyltransferaseN6AMT1cell morphologycell cyclecheckpointscyclinscytoskeletontubulinmitotic spindlemagistritöödThesis