Merilo,Ebe, juhendajaMorozova, DaanaTartu Ülikool. Loodus- ja täppisteaduste valdkondTartu Ülikool. Tehnoloogiainstituut2024-06-172024-06-172024https://hdl.handle.net/10062/99662Climate warming is associated with rising atmospheric Vapour Pressure Deficit (VPD), which affects plant physiology and production. Some proteins in the high VPD-induced stomatal closure pathway are known (e.g., protein kinase OST1), but there are still missing components. To address this knowledge gap, the study aimed to investigate Arabidopsis mutants defective in selected genes in order to reveal new elements that might be involved in VPD-induced stomata closure. Mutants were selected based on Wang et al. (2020) table of OST1 putative substrates and the expression levels of these genes in guard cells versus mesophyll cells. Experiments were conducted using a gas exchange measurement device to study plants’ stomatal conductance in response to high VPD and a plant stress hormone - abscisic acid (ABA). The study results showed no statistically significant differences in the steady-state stomata conductance and closure responses among mutants and wild-type, suggesting that these genes are not involved in VPD-induced stomata closure. Therefore, more studies are needed to reveal the missing components in the stomatal high VPD-induced closure pathway above OST1. Current results still add knowledge about stomatal behavior in future climatic conditions.enAttribution-NonCommercial-NoDerivs 3.0 EstoniaClimate changeVPDABAStomatal conductanceOST1bakalaureusetöödThesis