Generation of human papillomavirus type 11 and 18 genomes encoding hemagglutinin epitope-tagged E1 proteins

Date

2018

Journal Title

Journal ISSN

Volume Title

Publisher

Tartu Ülikool

Abstract

Human papillomaviruses (HPVs) are associated with numerous disorders ranging from warts to cancerous diseases. Although vaccination against several types of HPVs is available, a number of HPV-associated cancers remains stable. Therefore, studies on HPV are required for developing novel HPV-targeting drugs. Encoded by HPV E1 helicase is absolutely required for HPV replication. Therefore, it is a good target for developing antiviral drugs. This project aimed at generating HPV11 and HPV18 genomes encoding human influenza hemagglutinin (HA)-tagged E1 proteins with the further goal to test their replication ability in U2OS cell line. The study revealed that HA-tagged E1 proteins maintained the replication of the modified HPV genomes at levels similar to wild-type HPVs (wtHPVs). Thus, this study opens possibilities to investigate HPV11 and HPV18 endogenous E1 proteins using highly specific antibodies against HA epitope and introduces a less expensive and universal method to study E1-associated processes in the HPV life cycle.

Description

Keywords

E1 protein, HA-tag, human papillomavirus (HPV), replication

Citation