Generation of human papillomavirus type 11 and 18 genomes encoding hemagglutinin epitope-tagged E1 proteins
| dc.contributor.advisor | Piirsoo, Alla, juhendaja | et |
| dc.contributor.author | Levin, Viktor | |
| dc.contributor.other | Tartu Ülikool. Loodus- ja täppisteaduste valdkond | et |
| dc.contributor.other | Tartu Ülikool. Molekulaar- ja rakubioloogia instituut | et |
| dc.date.accessioned | 2018-08-14T08:19:47Z | |
| dc.date.available | 2018-08-14T08:19:47Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Human papillomaviruses (HPVs) are associated with numerous disorders ranging from warts to cancerous diseases. Although vaccination against several types of HPVs is available, a number of HPV-associated cancers remains stable. Therefore, studies on HPV are required for developing novel HPV-targeting drugs. Encoded by HPV E1 helicase is absolutely required for HPV replication. Therefore, it is a good target for developing antiviral drugs. This project aimed at generating HPV11 and HPV18 genomes encoding human influenza hemagglutinin (HA)-tagged E1 proteins with the further goal to test their replication ability in U2OS cell line. The study revealed that HA-tagged E1 proteins maintained the replication of the modified HPV genomes at levels similar to wild-type HPVs (wtHPVs). Thus, this study opens possibilities to investigate HPV11 and HPV18 endogenous E1 proteins using highly specific antibodies against HA epitope and introduces a less expensive and universal method to study E1-associated processes in the HPV life cycle. | et |
| dc.identifier.uri | http://hdl.handle.net/10062/61584 | |
| dc.language.iso | eng | et |
| dc.publisher | Tartu Ülikool | et |
| dc.rights | openAccess | et |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Estonia | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ee/ | * |
| dc.subject | E1 protein | et |
| dc.subject | HA-tag | et |
| dc.subject | human papillomavirus (HPV) | et |
| dc.subject | replication | et |
| dc.subject.other | bakalaureusetööd | et |
| dc.title | Generation of human papillomavirus type 11 and 18 genomes encoding hemagglutinin epitope-tagged E1 proteins | et |
| dc.type | Thesis | et |