The influence of menstrual cycle and endometriosis on endometrial methylome

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dc.contributor"European Union (EU)" and "Horizon 2020"
dc.contributor.authorSaare, Merli
dc.contributor.authorModhukur, Vijayachitra
dc.contributor.authorSuhorutshenko, Marina
dc.contributor.authorRajashekar, Balaji
dc.contributor.authorRekker, Kadri
dc.contributor.authorSõritsa, Deniss
dc.contributor.authorKarro, Helle
dc.contributor.authorSoplepmann, Pille
dc.contributor.authorSõritsa, Andrei
dc.contributor.authorLindgren, Cecilia M
dc.contributor.authorRahmioglu, Nilufer
dc.contributor.authorDrong, Alexander
dc.contributor.authorBecker, Christian M
dc.contributor.authorZondervan, Krina T
dc.contributor.authorSalumets, Andres
dc.contributor.authorPeters, Maire
dc.date.accessioned2019-02-26T10:53:51Z
dc.date.available2019-02-26T10:53:51Z
dc.date.issued2016-01
dc.description.abstractBACKGROUND: Alterations in endometrial DNA methylation profile have been proposed as one potential mechanism initiating the development of endometriosis. However, the normal endometrial methylome is influenced by the cyclic hormonal changes, and the menstrual cycle phase-dependent epigenetic signature should be considered when studying endometrial disorders. So far, no studies have been performed to evaluate the menstrual cycle influences and endometriosis-specific endometrial methylation pattern at the same time. RESULTS: Infinium HumanMethylation 450K BeadChip arrays were used to explore DNA methylation profiles of endometrial tissues from various menstrual cycle phases from 31 patients with endometriosis and 24 healthy women. The DNA methylation profile of patients and controls was highly similar and only 28 differentially methylated regions (DMRs) between patients and controls were found. However, the overall magnitude of the methylation differences between patients and controls was rather small (Δβ ranging from -0.01 to -0.16 and from 0.01 to 0.08, respectively, for hypo- and hypermethylated CpGs). Unsupervised hierarchical clustering of the methylation data divided endometrial samples based on the menstrual cycle phase rather than diseased/non-diseased status. Further analysis revealed a number of menstrual cycle phase-specific epigenetic changes with largest changes occurring during the late-secretory and menstrual phases when substantial rearrangements of endometrial tissue take place. Comparison of cycle phase- and endometriosis-specific methylation profile changes revealed that 13 out of 28 endometriosis-specific DMRs were present in both datasets. CONCLUSIONS: The results of our study accentuate the importance of considering normal cyclic epigenetic changes in studies investigating endometrium-related disease-specific methylation patterns.et
dc.identifier.urihttps://doi.org/10.1186/s13148-015-0168-z
dc.identifier.urihttp://hdl.handle.net/10062/63401
dc.language.isoenget
dc.publisherClin Epigeneticset
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/692065///WIDENLIFEet
dc.rightsinfo:eu-repo/semantics/openAccesset
dc.subjectDNA methylationet
dc.subjectEndometriosiset
dc.subjectEndometriumet
dc.subjectEpigeneticset
dc.subjectIllumina 450Ket
dc.subjectMenstrual cycleet
dc.subjectMicroarrayet
dc.titleThe influence of menstrual cycle and endometriosis on endometrial methylomeet
dc.typeinfo:eu-repo/semantics/articleet

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