dc.contributor	"European Union (EU)" and "Horizon 2020"
dc.contributor.author	Rekker, Kadri
dc.contributor.author	Altmäe, Signe
dc.contributor.author	Suhorutshenko, Marina
dc.contributor.author	Peters, Maire
dc.contributor.author	Martinez-Blanch, Juan F.
dc.contributor.author	Codoñer, Francisco M.
dc.contributor.author	Vilella, Felipe
dc.contributor.author	Simón, Carlos
dc.contributor.author	Salumets, Andres
dc.contributor.author	Velthut-Meikas, Agne
dc.date.accessioned	2019-02-27T08:46:18Z
dc.date.available	2019-02-27T08:46:18Z
dc.date.issued	2018
dc.description.abstract	The endometrium undergoes extensive changes to prepare for embryo implantation and microRNAs (miRNAs) have been described as playing a significant role in the regulation of endometrial receptivity. However, there is no consensus about the miRNAs involved in mid-secretory endometrial functions. We analysed the complete endometrial miRNome from early secretory (pre-receptive) and mid-secretory (receptive) phases from fertile women and from patients with recurrent implantation failure (RIF) to reveal differentially expressed (DE) miRNAs in the mid-secretory endometrium. Furthermore, we investigated whether the overall changes during early to mid-secretory phase transition and with RIF condition could be reflected in blood miRNA profiles. In total, 116 endometrial and 114 matched blood samples collected from two different population cohorts were subjected to small RNA sequencing. Among fertile women, 91 DE miRNAs were identified in the mid-secretory vs. early secretory endometrium, while no differences were found in the corresponding blood samples. The comparison of mid-secretory phase samples between fertile and infertile women revealed 21 DE miRNAs from the endometrium and one from blood samples. Among discovered novel miRNAs, chr2_4401 was validated and showed up-regulation in the mid-secretory endometrium. Besides novel findings, we confirmed the involvement of miR-30 and miR-200 family members in mid-secretory endometrial functions.	et
dc.identifier.uri	https://doi.org/10.3390/genes9120574
dc.identifier.uri	http://hdl.handle.net/10062/63429
dc.language.iso	eng	et
dc.relation	info:eu-repo/grantAgreement/EC/H2020/692065///WIDENLIFE	et
dc.relation.ispartofseries	Genes 2018, 9(12), 574;
dc.rights	info:eu-repo/semantics/openAccess	et
dc.subject	endometrial receptivity	et
dc.subject	infertility	et
dc.subject	microRNA	et
dc.subject	recurrent implantation failure	et
dc.subject	small RNA-seq	et
dc.title	A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women	et
dc.type	info:eu-repo/semantics/article	et

A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

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