(Tartu Ülikool, 2021) Agerova, Alissa; Tartu Ülikool. Loodus- ja täppisteaduste valdkond; Tartu Ülikool. Tehnoloogiainstituut
A eukaryotic cell contains a robust regulatory network that controls the ordered sequence
and timing of distinct cell cycle events. In budding yeast this is mainly regulated by the
growing activity of cyclin-dependent kinases (Cdks) during the cell cycle. Post-translational
modifications, particularly protein phosphorylation is a powerful mechanism regulating stability,
localization, activity and interactions of proteins. Building synthetic circuits based on
protein phosphorylation has remained a riddle due to the overlapping specificity of protein
kinases. To tackle this issue it was aimed to obtain a cell cycle independent cyclin-Cdk1
input that could target substrates created specifically for this particular cyclin-Cdk1 complex.
By manipulating the Cdk1 specificity of phospho-degron and -localization modules it
was anticipated to design orthogonal Cdk circuits that respond to the modified Clb5-Cdk1
complex.