The differences between cells in a population are often overlooked because they are obscured by the behavior of the entire population, however, the variation in the genome and transcriptome of every individual cell provides a comprehensive genetic insight. Single cell sorting is a robust technique that enables scientists to identify and sort individual cells for further downstream analysis or monoclonal cell culturing. The present study utilizes a BD FACSMelody cell sorter to sort different human cancer cells. The sorter’s efficiency as well as the viability of the sorted cells were assessed.