Sirvi Autor "Mets, Toomas, juhendaja" järgi

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Characterisation of Type II toxins discovered through iterative guilt-by-association search
(Tartu Ülikool, 2023) Rustamova, Aytan; Brodiazhenko, Tetiana, juhendaja; Mets, Toomas, juhendaja; Hauryliuk, Vasili, juhendaja
The classical toxin-antitoxin (TA) system is encoded as a bicistronic operon consisting of toxin and antitoxin genes. Toxin-antitoxin systems have been studied for more than 30 years and have been shown to play an important role in bacterial immunity and stress response. The most common and best-studied class of TA systems is type II, where both the toxin and the antitoxin are proteins, and the toxin is neutralized by the antitoxin via direct interaction. My thesis helps to uncover the molecular mechanisms of type II toxins found by the bioinformatics tool NetFlax (standing for Network of Flanking genes for toxins and antitoxins) via metabolic labelling assays. I characterize several toxins from different functional domains and show how the toxins affect the bacterial cell using Escherichia coli as the model organism.
Characterising a Novel HNH-Domain Containing Bacterial Immunity System From Escherichia coli
(Tartu Ülikool, 2025) Aliyeva, Elfi; Mets, Toomas, juhendaja; Abdullah, Minhal, juhendaja; Tartu Ülikool. Loodus- ja täppisteaduste valdkond; Tartu Ülikool. Bioinseneeria instituut
Bacteria are constantly in danger of being attacked by bacterial viruses - bacteriophages. To combat the threat, bacteria have developed a wide array of intricate and diverse immune systems. Phage therapy’s growing popularity has led to increased interest in bacterial immunity. My thesis investigates one such potential immunity system from Escherichia coli, discovered by our colleagues at Lund University, named Bogomol. It is predicted to be comprised of an HNH-containing endonuclease domain and a C-terminal domain of unknown function. I validated Bogomol as an immunity system acting against certain phages of Straboviridae. This was done by performing efficiency of plating assays and liquid culture experiments against the BASEL phage library. As an attempt to further investigate the triggers of Bogomol, escape mutant experiments were also conducted.
CsrA valgu ja ribonukleaaside osast toksiini-antitoksiini mRNA-de lagundamisel
(Tartu Ülikool, 2014-06-10) Tali, Aleksander; Kaldalu, Niilo, juhendaja; Mets, Toomas, juhendaja; Tartu Ülikool. Loodus- ja tehnoloogiateaduskond; Tartu Ülikool. Molekulaar- ja rakubioloogia instituut
Toksiin-antitoksiin süsteem relBE mõju E. coli rakkudele aminohappe nälja tingimustes
(Tartu Ülikool, 2018) Mäerand, Anett; Mets, Toomas, juhendaja; Kaldalu, Niilo,juhendaja; Tartu Ülikool. Loodus- ja täppisteaduste valdkond; Tartu Ülikool. Molekulaar- ja rakubioloogia instituut
Toksiin-antitoksiin (TA) süsteemid on geneetilised elemendid, mis koosnevad toksiinist ja antitoksiinist. Toksiinid inhibeerivad neid tootva bakteri kasvu ja antitoksiinid neutraliseerivad toksiine. Arvatakse, et TA süsteemid aktiveeruvad stressi korral ning aitavad bakterite kasvu seisata. Antud töö eesmärk oli uurida kas relBE, mazEF ja mqsRA toksiin-antitoksiin süsteemid mõjutavad Escherichia coli (E. coli) kasvu ja valguekspressiooni aminohapete nälja korral. Nägime, et eemaldatud relBE-ga BW25113 tüvi kasvas aminohappe näljas paremini kui metsiktüüpi tüvi. MG1655 ja Keio kollektsioonist pärit BW25113 ΔRelE tüves samas efekt puudus. MazF ja MqsR meie katse tingimustes aminohappe näljas kasvu ei mõjutanud. Aminohapete nälja tingimustes relBE rolli mõistmiseks on vaja täiendavaid katseid.