Sirvi Autor "Parik, Helen" järgi

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Mapping of ethyl methanesulfonate-induced mutations causing stomatal phenotypes
(Tartu Ülikool, 2020) Parik, Helen; Kollist, Hannes, juhendaja; Pandey, Ashutosh Kumar, juhendaja; Yarmolinsky, Dmitry, juhendaja; Tartu Ülikool. Loodus- ja täppisteaduste valdkond; Tartu Ülikool. Molekulaar- ja rakubioloogia instituut
Some aspects of stomatal regulation remain still unclear despite extensive research on finding and characterizing stomatal regulators. As stomata are responsible for controlling plant water loss, the interest to better our understanding of stomatal regulation is constantly growing, as fresh water availability is getting scarce and CO2 and tropospheric O3 levels in the atmosphere are increasing. This study contributes to a large-scale forward genetic screen aimed to identify missing links in stomatal regulation pathways by collection of plant material for mapping, identifying a causal mutation for a phenotype and verifying mapped stomatal regulators. This work identified SDD1 and CTL1 to be involved in stomatal regulation
Stomatal Conductance Depends on Abscisic Acid Production in Both Guard Cells and in Phloem Companion Cells
(Tartu Ülikool, 2017-06-10) Parik, Helen; PhD, Dmitry Yarmolinsky, supervisor; Prof. Hannes Kollist , supervisor; Tartu Ülikool. Loodus- ja täppisteaduste valdkond; Tartu Ülikool. Molekulaar- ja rakubioloogia instituut
The question how plants use water is important as water availability is often a limiting factor for plant growth. Stomata are mainly responsible for water loss from plant tissues and, therefore, regulatory mechanisms controlling stomatal apertures are in the research focus of plant water management. Abscisic acid (ABA) has an important role in reducing stomatal conductance, however, importance of ABA biosynthesis in guard cells and in phloem is not fully studied. To address this question, we generated transgenic plants with restored ABA biosynthesis either in guard cells or in phloem by using tissue-specific promoters. Our study shows that both guard cells and phloem companion cells can produce ABA in quantities which are enough to regulate plant development and stomatal conductance on the level of wildtype plants.