Browsing by Author "Tark, Mariliis"

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DNA reparatsioon Pseudomonas putida rakkudes
(2006) Tark, Mariliis; Tover, Andres, juhendaja; Kivisaar, Maia, juhendaja
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Mutagenic potential of DNA damage repair and tolerance mechanisms under starvation stress
(2009-10-21T08:49:55Z) Tark, Mariliis
Upon being confronted with DNA damage, bacteria, as any other cell, have two options for survival: either to repair the DNA or to tolerate the damage. If neither of the two succeeds and the lesions remain in the DNA, the cell dies. There are several partially redundant repair pathways to cope with damage, as well as several specialized DNA polymerases to carry out replication across lesions in DNA. In general, DNA repair and damage tolerance due to the action of specialized DNA polymerases are temporally separated processes. First, repair of damage, an intrinsically error-free process is initiated. If damage is not repaired within a certain time, the specialized, often error-prone DNA polymerases are allowed to save the cell from the worst. The best and probably most studied example of these ‘sequential’ repair and tolerance is the way bacteria deal with UV-induced DNA damage. I have here addressed the influence of mechanisms of DNA repair and tolerance on mutational processes in bacteria under conditions of carbon starvation. The energy status of bacteria in these conditions is low and chromosome replication and bacterial division are strongly reduced. I have investigated Pseudomonas putida pathways that deal with UV-induced damage: nucleotide excision repair and the activity of specialized DNA polymerases.