Sirvi Märksõna "antibiotic residue analysis" järgi

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Determination of antibacterials in river water by solid phase extraction using LC -MS/MS
(Tartu Ülikool, 2020) Iftikhar, Waseem Ahmad; Herodes, Koit; Tartu Ülikool. Loodus- ja täppisteaduste valdkond
Analytical method was developed for the detection of fluoroquinolones (FQs), sulfonamides and Amphenicol in Emajogi river in Tartu City. The compounds were simultaneously extracted from river water using solid phase extraction (SPE). Identification and quantification was done through Liquid chromatography – tandem mass spectrometry (LC-MS/MS) in selected reaction monitoring (MRM) mode.The recovery of FQs ranged 62% for Enrofloxacin to 76% Marbofloxacin and 68% Florfenicol ,69% sulfamethoxazole. Limit of Quantification ranged from 0.1 ngg-1 for SA’s ,0.6 - 1.5 ngg-1 for FQ’s, and 0.9ngg-1 for FF. The method was developed and valdated for river water analysis of samples from upstream, midpoint and down stream of the WWTP. The method developed may be used for a more in-depth study on the occurence and fate of these commonly used pharmaceuticals in river water bodies.
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Simultaneous determination of selected antimicrobial agents in sewage sludge by pressurized liquid extraction and LC -MS/MS
(Tartu Ülikool, 2020) Osagu, Joshua Onyeka; Herodes, Koit; Tartu Ülikool. Loodus- ja täppisteaduste valdkond
In the present study, an efficient analytical method for the simultaneous determination of an amphenicol, sulfonamides (SAs) and fluoroquinolones (FQs) in a sewage sludge matrix was developed by high performance liquid chromatography in tandem with mass spectrometry (LCMS). The selected antibiotics were extracted from the sewage sludge sample by pressurized liquid extraction (PLE) followed by solid phase extraction (SPE) as a clean-up step. Compounds separation was achieved using a mobile phase gradient composition of hexafluoro-isopropanol buffer and methanol. Recoveries of all eight compounds were in the range from 69% to 166%. Limit of quantification ranged from 0.6 ng/g for FQs to 9.3 ng/g for SAs. As most of the compounds showed significant matrix effects, the method was validated using the standard addition method which reduced this effect very significantly.