Sirvi Märksõna "chemical imaging" järgi

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Extending the Depth of Focus of Infrared Microscope Using a Binary Axicon Fabricated on Barium Fluoride
(2024) Han, Molong; Smith, Daniel; Kahro, Tauno; Stonytė, Dominyka; Kasikov, Aarne; Gailevičius, Darius; Tiwari, Vipin; Xavier, Agnes Pristy Ignatius; Gopinath, Shivasubramanian; Ng, Soon Hock; Rajeswary, Aravind Simon John Francis; Tamm, Aile; Kukli, Kaupo
Axial resolution is one of the most important characteristics of a microscope. In all microscopes, a high axial resolution is desired in order to discriminate information efficiently along the longitudinal direction. However, when studying thick samples that do not contain laterally overlapping information, a low axial resolution is desirable, as information from multiple planes can be recorded simultaneously from a single camera shot instead of plane-by-plane mechanical refocusing. In this study, we increased the focal depth of an infrared microscope non-invasively by introducing a binary axicon fabricated on a barium fluoride substrate close to the sample. Preliminary results of imaging the thick and sparse silk fibers showed an improved focal depth with a slight decrease in lateral resolution and an increase in background noise.
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Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam
(Licensee MDPI, 2022) Han, Molong; Smith, Daniel; Ng, Soon Hock; Katkus, Tomas; Rajeswary, Aravind Simon John Francis; Praveen, Periyasamy Angamuthu; Bambery, Keith R.; Tobin, Mark J.; Vongsvivut, Jitraporn; Juodkazis, Saulius; Anand, Vijayakumar
Phase imaging of biochemical samples has been demonstrated for the first time at the Infrared Microspectroscopy (IRM) beamline of the Australian Synchrotron using the usually discarded near-IR (NIR) region of the synchrotron-IR beam. The synchrotron-IR beam at the Australian Synchrotron IRM beamline has a unique fork shaped intensity distribution as a result of the gold coated extraction mirror shape, which includes a central slit for rejection of the intense X-ray beam. The resulting beam configuration makes any imaging task challenging. For intensity imaging, the fork shaped beam is usually tightly focused to a point on the sample plane followed by a pixel-by-pixel scanning approach to record the image. In this study, a pinhole was aligned with one of the lobes of the fork shaped beam and the Airy diffraction pattern was used to illuminate biochemical samples. The diffracted light from the samples was captured using a NIR sensitive lensless camera. A rapid phase-retrieval algorithm was applied to the recorded intensity distributions to reconstruct the phase information. The preliminary results are promising to develop multimodal imaging capabilities at the IRM beamline of the Australian Synchrotron.