Phospho-regulation of nuclear localization modules by Cdk1
Date
2021
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Abstract
The cells are subjects to many internal events such as the cell cycle which results in cell
proliferation. The cell cycle events are regulated by protein phosphorylation done by cyclindependent
kinases (CDKs) which target specific targets in well-timed manner using binding
partners known as cyclins and cyclin-dependent protein kinase regulatory subunits. The
changes induced by such complexes can affect protein subcellular localization by activating
or inhibiting nuclear localization sequences (NLS) and nuclear export signals (NES) - amino
acid regions, recognized by dedicated shuttling proteins. In this thesis the influence of cyclin
specificity on protein nuclear localization patterns in Cdk1 targets, containing cyclin
docking regions, is studied. Specific experiments are conducted on Dna2(1-100) and
Psy4(315-441) which are two truncated modules of original proteins known for subcellular
localization dependence on the cell cycle.
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Keywords
Cell cycle, Cellular localization, Phosphorylation, Cyclin-dependent kinase