Isolation and characterization of a phage collection against Pseudomonas putida

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dc.contributor.authorBrauer, Age
dc.contributor.authorRosendahl, Sirli
dc.contributor.authorKängsep, Anu
dc.contributor.authorLewańczyk, Alicja Cecylia
dc.contributor.authorRikberg, Roger
dc.contributor.authorHõrak, Rita
dc.contributor.authorTamman, Hedvig
dc.date.accessioned2024-08-05T09:17:50Z
dc.date.available2024-08-05T09:17:50Z
dc.date.issued2024-06-11
dc.descriptionAutorite ORCID: Age Brauer https://orcid.org/0000-0003-4779-2458 Sirli Rosendahl https://orcid.org/0009-0005-7403-3862 Alicja Cecylia Lewańcyk https://orcid.org/0009-0001-4600-2277 Rita Hõrak https://orcid.org/0000-0002-3828-6281 Hedvig Tamman https://orcid.org/0000-0003-4453-7814
dc.description.abstractThe environmental bacterium, Pseudomonas putida, possesses a broad spectrum of metabolic pathways. This makes it highly promising for use in biotechnological production as a cell factory, as well as in bioremediation strategies to degrade various aromatic pollutants. For P. putida to flourish in its environment, it must withstand the continuous threats posed by bacteriophages. Interestingly, until now, only a handful of phages have been isolated for the commonly used laboratory strain, P. putida KT2440, and no phage defence mechanisms have been characterized. In this study, we present a new Collection of Environmental P. putida Phages from Estonia, or CEPEST. This collection comprises 67 double-stranded DNA phages, which belong to 22 phage species and 9 phage genera. Our findings reveal that most phages in the CEPEST collection are more infectious at lower temperatures, have a narrow host range, and require an intact lipopolysaccharide for P. putida infection. Furthermore, we show that cryptic prophages present in the P. putida chromosome provide strong protection against the infection of many phages. However, the chromosomal toxin–antitoxin systems do not play a role in the phage defence of P. putida. This research provides valuable insights into the interactions between P. putida and bacteriophages, which could have significant implications for biotechnological and environmental applications.
dc.description.sponsorship„Deciphering stringent response proteins and toxin-antitoxin systems in the arms race between bacteria and phages“ PhaBacArms, ERC-StG grant No. 101116205
dc.description.sponsorshipEstonian Research Council with EMBO „The role of stringent response in the phage defence of Pseudomonas putida“ EMBO IG-5323-2023
dc.description.sponsorshipEstonian Research Council: „Chromosomal Toxin-Antitoxin Systems – a Mechanism to Resist Phage Infection?“ PRG1431 and „The role of alarmone ppGpp in phage-resistance of Pseudomonas putida“ MOBTP1017
dc.identifier.urihttps://doi.org/10.1111/1462-2920.16671
dc.identifier.urihttps://hdl.handle.net/10062/102107
dc.language.isoen
dc.relation.ispartofEnvironmental Microbiology, Volume 26, Issue 6
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleIsolation and characterization of a phage collection against Pseudomonas putida
dc.typeArticle

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