Differentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development: The Plausible Role of miR-139-5p and miR-375

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dc.contributor"European Union (EU)" and "Horizon 2020"
dc.contributor.authorRekker, Kadri
dc.contributor.authorTasa, Tõnis
dc.contributor.authorSaare, Merli
dc.contributor.authorSamuel, Külli
dc.contributor.authorKadastik, Ülle
dc.contributor.authorKarro, Helle
dc.contributor.authorGötte, Martin
dc.contributor.authorSalumets, Andres
dc.contributor.authorPeters, Maire
dc.date.accessioned2019-02-27T09:06:20Z
dc.date.available2019-02-27T09:06:20Z
dc.date.issued2018
dc.description.abstractmicroRNA (miRNA) expression level alterations between endometrial tissue and endometriotic lesions indicate their involvement in endometriosis pathogenesis. However, as both endometrium and endometriotic lesions consist of different cell types in various proportions, it is not clear which cells contribute to variability in miRNA levels and the overall knowledge about cell-type specific miRNA expression in ectopic cells is scarce. Therefore, we utilized fluorescence-activated cell sorting to isolate endometrial stromal cells from paired endometrial and endometrioma biopsies and combined it with high-throughput sequencing to determine miRNA alterations in endometriotic stroma. The analysis revealed 149 abnormally expressed miRNAs in endometriotic lesions, including extensive upregulation of miR-139-5p and downregulation of miR-375 compared to eutopic cells. miRNA transfection experiments in the endometrial stromal cell line ST-T1b showed that the overexpression of miR-139-5p resulted in the downregulation of homeobox A9 (HOXA9) and HOXA10 expression, whereas the endothelin 1 (EDN1) gene was regulated by miR-375. The results of this study provide further insights into the complex molecular mechanisms involved in endometriosis pathogenesis and demonstrate the necessity for cell-type-specific analysis of ectopic tissues to understand the interactions between different cell populations in disease onset and progression.et
dc.identifier.urihttps://doi.org/10.3390/ijms19123789
dc.identifier.urihttp://hdl.handle.net/10062/63431
dc.language.isoenget
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/692065///WIDENLIFEet
dc.relation.ispartofseriesInt. J. Mol. Sci. 2018;19(12), 3789
dc.rightsinfo:eu-repo/semantics/openAccesset
dc.subjectendometriosiset
dc.subjectectopic stromaet
dc.subjectmicroRNAet
dc.subjectsmall RNA sequencinget
dc.subjectEDN1et
dc.subjectHOXA10et
dc.subjectmiR-139-5pet
dc.subjectmiR-375et
dc.titleDifferentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development: The Plausible Role of miR-139-5p and miR-375et
dc.typeinfo:eu-repo/semantics/articleet

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