Karyotype of the blastocoel fluid demonstrates low concordance with both trophectoderm and inner cell mass

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dc.contributor"European Union (EU)" and "Horizon 2020"
dc.contributor.authorTšuiko, Olga
dc.contributor.authorZhigalina, Daria I.
dc.contributor.authorJatsenko, Tatjana
dc.contributor.authorSkryabin, Nikolay A.
dc.contributor.authorKanbekova, Olga R.
dc.contributor.authorArtyukhova, Victoria G.
dc.contributor.authorSvetlakov, Anatoly V.
dc.contributor.authorTeearu, Katre
dc.contributor.authorTrošin, Aleksander
dc.contributor.authorSalumets, Andres
dc.contributor.authorKurg, Ants
dc.contributor.authorLebedev, Igor N.
dc.dateinfo:eu-repo/date/embargoEnd/2019-07-01
dc.date.accessioned2019-02-26T14:32:13Z
dc.date.available2019-02-26T14:32:13Z
dc.date.issued2018-06
dc.description.abstractObjective To compare the genomic profiles of blastocoel fluid (BF), inner cell mass (ICM), and trophectoderm (TE) cells derived from the same blastocyst. Design Prospective study. Setting Academic and in vitro fertilization units. Patient(s) Sixteen donated cryopreserved embryos at blastocyst stage. Intervention(s) BF, TE, and ICM cells were retrieved from each blastocyst for chromosome analysis by means of next-generation sequencing (NGS). Main Outcome Measure(s) Aneuploidy screening and assessment of mosaicism in BF, TE and ICM samples with subsequent comparison of genomic profiles between the three blastocyst compartments. Result(s) Out of 16 blastocysts, 10 BF samples and 14 TE and ICM samples provided reliable NGS data for comprehensive chromosome analysis. Only 40.0% of BF-DNA karyotypes were fully concordant with TE or ICM, compared with 85.7% concordance between TE and ICM. In addition, BF-DNA was burdened with mosaic aneuploidies and the total number of affected chromosomes in BF was significantly higher compared with the TE and ICM. Conclusion(s) BF-DNA can be successfully amplified and subjected to NGS, but owing to increased discordance with ICM and TE, BF does not adequately represent the status of the rest of the embryo. To overcome biologic and technical challenges associated with BF sampling and processing, blastocentesis would require improvement in both laboratory protocols and aneuploidy calling algorithms. Therefore, TE biopsy remains the most effective way to predict embryonic karyotype, and the use of BF as a single source of DNA for preimplantation genetic screening is not yet advised.et
dc.identifier.urihttps://doi.org/10.1016/j.fertnstert.2018.02.008
dc.identifier.urihttp://hdl.handle.net/10062/63420
dc.language.isoenget
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/692065///WIDENLIFEet
dc.relation.ispartofseriesFertility and Sterility;2018 Jun;109(6):1127-1134.e1.
dc.rightsinfo:eu-repo/semantics/embargoedAccesset
dc.subjectBlastocentesispreimplantation genetic screeninget
dc.subjectmosaicismet
dc.subjectblastocoel fluidet
dc.subjectnext-generation sequencinget
dc.titleKaryotype of the blastocoel fluid demonstrates low concordance with both trophectoderm and inner cell masset
dc.typeinfo:eu-repo/semantics/articleet

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