Regulation of the cell cycle to cytoskeleton remodelling: the diverse functions of N6AMT1
Date
2023
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Tartu Ülikool
Abstract
N6AMT1, also known as HEMK2, is an evolutionally conserved protein which belongs to the
human TRMT112-methyltransferase network. It is involved in the methylation of various
substrates. Previous studies have shown that this significant methyltransferase is engaged in the
majority of vital processes like regulating of cell proliferation, cell cycle factors, etc. Therefore,
in the present research, it was studied how the depletion of N6AMT1 affected the viability of
U2OS cells. Using CRISPR/Cas9 genome editing system we obtained an N6AMT1 knock-out
cell line that showed stunted growth, delayed cell cycle progression, and alterations in
morphology. Moreover, it was noticed that N6AMT1 reduction had an impact on cytoskeleton
remodelling, namely the β-tubulin level. In conclusion, human N6AMT1 was considered as a
potential target responsible for cell cycle and cell cytoskeleton remodelling.
Description
Keywords
methyltransferase, N6AMT1, cell morphology, cell cycle, checkpoints, cyclins, cytoskeleton, tubulin, mitotic spindle