Characterization of Human MAGE-A10 protein

Abstract

MAGE-A10, the most antigenic protein member of the MAGE-A family of cancer-testis antigen (CTAs), is expressed in tumors but not in normal tissues except for the testis, fetal ovary, and placenta. Their tumor-restricted expression pattern makes these proteins an at tractive target for cancer immunotherapy. The computational molecular weight of MAGE A10 is ~41kDa. However, in the gel, it runs at ~72kDa. In this research, COP5-EBNA cells were transfected with plasmid DNA encoding for different stretches of the MAGE-A10 pro tein, and the size anomalies and localization of the proteins were determined. Our results suggested that the N-terminal region of the protein is responsible for the size anomalies and nuclear localization of the MAGE-A10 protein.