T-cell receptor repertoire analysis in CVID patients post Comirnaty vaccination

dc.contributor.advisorKisand, Kai, juhendaja
dc.contributor.advisorElsakova, Alexandra, juhendaja
dc.contributor.authorAghayari, Avishan
dc.contributor.otherTartu Ülikool. Loodus- ja täppisteaduste valdkondet
dc.contributor.otherTartu Ülikool. Tehnoloogiainstituutet
dc.date.accessioned2024-06-19T07:52:10Z
dc.date.available2024-06-19T07:52:10Z
dc.date.issued2024
dc.description.abstractCommon variable immunodeficiency (CVID) is one the most diagnosed primary immunodeficiencies (PID) with a complex etiology, characterized by low levels of serum immunoglobulins mainly due to B-cell deficiencies with potential T-cell dysregulations and abnormalities. This immune system dysfunction puts CVID patients at a higher risk for developing severe infections, including COVID-19. Despite its critical importance, their immune response to SARS-CoV-2 infection and vaccination remains unclear. Studying the T-cell-receptor (TCR) repertoire of these patients sheds light on the dynamics of their cellular immune response, knowing that T-cells recognize antigens through their TCR. In this study, we utilized high throughput sequencing to investigate and profile the TCR repertoire of PID patients, particularly those with CVID following the second dose of the Pfizer-BioNTech Comirnaty-BNT162b2 vaccine. We report on the diversity and clonality of TCR repertoires between diseased and healthy individuals post-vaccination. Investigating gene segment usage, we found a bias in the frequency of TCR V beta gene segment usage between patients and controls. Lastly, we examined the clonal expansion of T-cells following Spike-antigen (S Ag) stimulation to provide insights into the T-cell response post-vaccination.
dc.identifier.urihttps://hdl.handle.net/10062/100023
dc.language.isoen
dc.publisherTartu Ülikoolet
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Estoniaen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/ee/
dc.subjectT-cell receptor repertoire
dc.subjectCVID
dc.subjectPID
dc.subjectCOVID-19
dc.subjectVaccination
dc.subject.othermagistritöödet
dc.titleT-cell receptor repertoire analysis in CVID patients post Comirnaty vaccination
dc.title.alternativeT-raku retseptorite repertuaari analüüs üldise variaabli immuunpuudulikkusega patsientidel Comirnaty vaktsiini järgselt
dc.typeThesisen

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