Functional studies on the formation of Chikungunya virus replicase through the trans-complementation of defected replicase components
| dc.contributor.advisor | Lello, Laura Sandra | |
| dc.contributor.author | Claesson, Hanna Carolina | |
| dc.date.accessioned | 2023-06-15T12:42:19Z | |
| dc.date.available | 2023-06-15T12:42:19Z | |
| dc.date.issued | 2023 | |
| dc.description.abstract | Chikungunya virus has a positive-strand RNA genome and belongs to the Alphavirus genus. The viral replication complex includes four non-structural proteins (nsP1-4) which are needed for the virus replication. Several mutations in the non-structural region have been described which impair the formation and functioning of the replication complexes. The alphavirus replicase possesses trans activity which has enabled for the construction of trans-replication systems. These systems provide efficient tools for studying the replication of alphaviruses. The aim of this study was to explore the possibility of trans-complementing the impaired functions of CHIKV nsP1, nsP2 and nsP3 proteins by supplying a functional counterpart in trans. This thesis demonstrated that the inactivity of the CHIKV replicases can be, in some cases, trans-complemented. | et |
| dc.identifier.uri | https://hdl.handle.net/10062/90664 | |
| dc.language.iso | eng | et |
| dc.publisher | Tartu Ülikool | et |
| dc.rights | embargoedAccess | et |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.subject | Chikungunya virus | et |
| dc.subject | trans-complementation | et |
| dc.subject | trans-replication | et |
| dc.subject | non-structural protein | et |
| dc.title | Functional studies on the formation of Chikungunya virus replicase through the trans-complementation of defected replicase components | et |
| dc.type | Thesis | et |