Prevalence of Apo A and Apo E in extracellular vesicles using different purification methods

Abstract

Apolipoprotein (Apo), a protein portion of plasma lipoprotein, can attach to and distribute blood lipids to different tissues in the body for metabolism and utilization. It is primarily synthesized in the liver and, to a lesser extent, in the small intestine. High levels of apolipo-proteins may affect blood lipid metabolism and use, causing the onset and progression of hyperlipidemia and atherosclerosis that might lead to cardiovascular and cerebrovascular disease. However, these lipid-protein molecules are also present in extracellular vesicles (EVs) samples. The study shows various extracellular vesicle purification methods to detect apolipoproteins as well as how the apolipoprotein yield correlates with apolipoproteins and cholesterol fractions measured by routine methods in a clinical laboratory.