Utilising FGF2, IGF2 and FSH in serum-free protocol for long-term in vitro cultivation of primary human granulosa cells

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dc.contributor.authorHensen, Kati
dc.contributor.authorPook, Martin
dc.contributor.authorSikut, Anu
dc.contributor.authorOrg, Tõnis
dc.contributor.authorMaimets, Toivo
dc.contributor.authorSalumets, Andres
dc.contributor.authorKurg, Ants
dc.date.accessioned2020-04-22T10:35:07Z
dc.date.available2020-04-22T10:35:07Z
dc.date.issued2020
dc.description.abstractHuman granulosa cells acquired as leftover from IVF treatment can be used to study infertility problems and area valuable tool in the research of follicle maturation and ovulation. There is a need for more defined and long-term culture protocols for studying the response of granulosa cells upon treatment with selected hormones/chemicals. In the current study, we tested the effect of adding FGF2, IGF2 and FSH into defined basal medium inorder tofind culture conditions that would support proliferation of cumulus and mural granulosa cells alongwith the expression of common granulosa cell type markers such asFSHR,AMHR2,LHRandCYP19A1. We foundthat FGF2, IGF2 together with FSH helped to retain granulosa cell marker expression while supporting cellsurvival at least for two weeks of culture. The defined serum-free culture conditions for long-term culturing willbe valuable in providing new standards in the research of human granulosa cells.et
dc.identifier.urihttps://doi.org/10.1016/j.mce.2020.110816
dc.identifier.urihttp://hdl.handle.net/10062/67330
dc.language.isoenget
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/692065///WIDENLIFE
dc.relation.ispartofseriesMolecular and Cellular Endocrinology;Volume 510
dc.rightsinfo:eu-repo/semantics/openAccesset
dc.subjectHuman granulosa cellset
dc.subjectCell cultureet
dc.subjectFSHet
dc.subjectFSHRet
dc.subjectFGF2et
dc.subjectIGF2et
dc.titleUtilising FGF2, IGF2 and FSH in serum-free protocol for long-term in vitro cultivation of primary human granulosa cellset
dc.typeinfo:eu-repo/semantics/articleet

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