Stable expression of Barmah Forest virus replicons in cultured mammalian cells
Date
2023
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Tartu Ülikool
Abstract
Viruses are infectious agents that can infect various living organisms, including humans,
animals, plants, and microorganisms. Understanding their molecular biology is crucial for
developing effective prevention and treatment strategies. Alphaviruses are a group of
mosquito-borne viruses that cause diseases such as rash, arthritis, and encephalitis. Barmah
Forest virus (BFV) is an alphavirus that poses a public health threat in Australia. However,
research on BFV is relatively limited compared to other alphaviruses. In this study, we
focus on the expression of BFV replicons in cultured mammalian cells. Replicons are
valuable tools for studying viral replication and developing antiviral agents. We review the
successful generation of replicons for alphaviruses and Hepatitis C virus and propose a
workflow for constructing BFV replicons in cultured mammalian cells.
Description
Keywords
alphavirus, Barmah Forest virus, alphavirus replicon, Barmah Forest virus replicon, stable cell lines