Primary screening of co-factors involving Scribble-mediated growth regulation in the Drosophila wing imaginal disc
Date
2024
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Tartu Ülikool
Abstract
The wing imaginal disc of Drosophila melanogaster has been used as a model to study tissue
growth and morphogenesis. This disc is composed of epithelial cells exhibiting apical-basal
polarity (APB), which is essential for tissue development and homeostasis. Disruption of APB
can lead to a loss in cell polarity that may result in neoplasia formation. Scribble (Scrib) is
one of the key components involved in maintenance of ABP. Recent studies indicate that the
copy number of scrib in the tissue directly influences formation of neoplasia when scrib is
conditionally knocked down using an RNAi approach. This leads to the hypothesis that there
is a synergy between additional genes and Scrib in the Scrib-derived neoplasia formation. To
test this hypothesis, gene screening was performed. Deficiency fly lines with a specific
deleted region and a conditional knock-down of scrib initiated by RNAi were used to find
genes of interest. Following the evaluation of two different protocols, possible candidates for
further screening were found.
Description
Keywords
Drosophila, ABP, Scribble, Gene screening, Deficiency line, Wing imaginal disc