Determination of Amino Acids in Bee Products by Diethyl ethoxymethylenemalonate Derivatization Using LC-ESI-MS/MS

dc.contributor.advisorHerodes, Koit
dc.contributor.authorBùi, Nguyên Kim Ngân
dc.contributor.otherTartu Ülikool. Loodus- ja täppisteaduste valdkondet
dc.date.accessioned2019-09-26T09:29:58Z
dc.date.available2019-09-26T09:29:58Z
dc.date.issued2019
dc.description.abstractAmino acid content of honey and royal jelly has been used for the product authentication, geographical and botanical origin evaluation, indication of freshness and nutritional value. In this thesis analytical method was tested and improved for analysis of the concentration of free amino acids in Vietnamese monofloral (Robusta coffee) honey and Royal Jelly by derivatization with diethyl ethoxymethylenemalonate (DEEMM) using liquid chromatography coupled to mass spectrometry (LC-MS/MS). The derivatization proceeds under aqueous conditions and is sufficiently fast reaction for most amino acids. Limits of quantitation at ng g-1 level were achieved for most amino acids. The method has good repeatability and recovery in general. Determined amino acid contents agree, in general, well with literature findings in different honey and royal jelly samples. The differences found may be indicative of botanical/geographical origin of these bee products.et
dc.identifier.urihttp://hdl.handle.net/10062/65333
dc.language.isoenget
dc.publisherTartu Ülikoolet
dc.rightsopenAccesset
dc.rightsAutorile viitamine + Mitteäriline eesmärk + Tuletatud teoste keeld 3.0 Eesti*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/ee/*
dc.subjectAmino Acidset
dc.subjectRobusta coffeeet
dc.subjecthoneyet
dc.subjectRoyal Jellyet
dc.subjectLC-MS/MSet
dc.titleDetermination of Amino Acids in Bee Products by Diethyl ethoxymethylenemalonate Derivatization Using LC-ESI-MS/MSet
dc.typeThesiset

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