Globin mRNA reduction for whole-blood transcriptome sequencing

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dc.contributor"European Union (EU)" and "Horizon 2020"
dc.contributor.authorKrjutškov, Kaarel
dc.contributor.authorKoel, Mariann
dc.contributor.authorRoost, Anne Mari
dc.contributor.authorKatayama, Shintaro
dc.contributor.authorEinarsdottir, Elisabet
dc.contributor.authorJouhilahti, Eeva-Mari
dc.contributor.authorSöderhäll, Cilla
dc.contributor.authorJaakma, Ülle
dc.contributor.authorPlaas, Mario
dc.contributor.authorVesterlund, Liselotte
dc.contributor.authorLohi, Hannes
dc.contributor.authorSalumets, Andres
dc.contributor.authorKere, Juha
dc.date.accessioned2019-02-26T12:27:44Z
dc.date.available2019-02-26T12:27:44Z
dc.date.issued2016
dc.description.abstractThe transcriptome analysis of whole-blood RNA by sequencing holds promise for the identification and tracking of biomarkers; however, the high globin mRNA (gmRNA) content of erythrocytes hampers whole-blood and buffy coat analyses. We introduce a novel gmRNA locking assay (GlobinLock, GL) as a robust and simple gmRNA reduction tool to preserve RNA quality, save time and cost. GL consists of a pair of gmRNA-specific oligonucleotides in RNA initial denaturation buffer that is effective immediately after RNA denaturation and adds only ten minutes of incubation to the whole cDNA synthesis procedure when compared to non-blood RNA analysis. We show that GL is fully effective not only for human samples but also for mouse and rat, and so far incompletely studied cow, dog and zebrafish.et
dc.identifier.urihttps://doi.org/10.1038/srep31584
dc.identifier.urihttp://hdl.handle.net/10062/63407
dc.language.isoenget
dc.publisherScientific Reportset
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/692065///WIDENLIFEet
dc.relation.ispartofseriesScientific Reports;6, 31584−31584
dc.rightsinfo:eu-repo/semantics/openAccesset
dc.subjectGene expressionet
dc.subjectRNA sequencinget
dc.titleGlobin mRNA reduction for whole-blood transcriptome sequencinget
dc.typeinfo:eu-repo/semantics/articleet

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