Sirvi Kuupäev , alustades "2018-06-01" järgi

Filtreeri tulemusi aasta või kuu järgi

Nüüd näidatakse 1 - 3 3

Karyotype of the blastocoel fluid demonstrates low concordance with both trophectoderm and inner cell mass
(2018-06) Tšuiko, Olga; Zhigalina, Daria I.; Jatsenko, Tatjana; Skryabin, Nikolay A.; Kanbekova, Olga R.; Artyukhova, Victoria G.; Svetlakov, Anatoly V.; Teearu, Katre; Trošin, Aleksander; Salumets, Andres; Kurg, Ants; Lebedev, Igor N.
Objective To compare the genomic profiles of blastocoel fluid (BF), inner cell mass (ICM), and trophectoderm (TE) cells derived from the same blastocyst. Design Prospective study. Setting Academic and in vitro fertilization units. Patient(s) Sixteen donated cryopreserved embryos at blastocyst stage. Intervention(s) BF, TE, and ICM cells were retrieved from each blastocyst for chromosome analysis by means of next-generation sequencing (NGS). Main Outcome Measure(s) Aneuploidy screening and assessment of mosaicism in BF, TE and ICM samples with subsequent comparison of genomic profiles between the three blastocyst compartments. Result(s) Out of 16 blastocysts, 10 BF samples and 14 TE and ICM samples provided reliable NGS data for comprehensive chromosome analysis. Only 40.0% of BF-DNA karyotypes were fully concordant with TE or ICM, compared with 85.7% concordance between TE and ICM. In addition, BF-DNA was burdened with mosaic aneuploidies and the total number of affected chromosomes in BF was significantly higher compared with the TE and ICM. Conclusion(s) BF-DNA can be successfully amplified and subjected to NGS, but owing to increased discordance with ICM and TE, BF does not adequately represent the status of the rest of the embryo. To overcome biologic and technical challenges associated with BF sampling and processing, blastocentesis would require improvement in both laboratory protocols and aneuploidy calling algorithms. Therefore, TE biopsy remains the most effective way to predict embryonic karyotype, and the use of BF as a single source of DNA for preimplantation genetic screening is not yet advised.
Kaitseväe ajateenijate kehaline aktiivsus, alajäsemete vigastused ja põlveliigese valu: 2014-2016 aasta andmete analüüs
(Tartu Ülikool, 2018-06-01) Seppa, Andre
Aim: The aim of the study was to investigate physical activity, lower extremities injuries, and knee joint pain in conscripts as well as to find assosications between measured characteristics. Methods: Total 256 male conscripts aged of 18-27 of Kuperjanov Infantry Battalion, Estonian Defence Forces who entered military service in 2014 autumn (Oct. 2014), 2015 summer (Jul. 2015) and 2016 autumn (Oct. 2016) participated in the cross-sectional study used for this masters thesis. Questionaire for previous injuries of low extremities and sports related activity, Baecke habitual physical activity (PA) questionaire and Knee Injury and Osteoarthritis Outcome Score (KOOS) questionaire were used. Spearman’s correlation coefficients were calculated. Results: The Body mass index (BMI) of the conscripts was (mean±SD) 23,9±3,0 kg/m², BMI greater than 25 kg/m² was for 33,2%. 77,8% of conscripts participated in some sort of sports activity, mean period of sport activity was 6,6±3,9 year and training load was 7,5±5,5 hours per week. At least 48,4% of conscripts had one lower extremity injury before military service. Baecke questionaire PA work-related scores were 2,97±0,78; sport-related scores were 2,31±1,60; leisure time related scores were 3,07±0,62; total score was 8,50±1,92. KOOS questionaire mean scores were calculated for subscale of symptoms83,0±14,9; pain 87,6±15,1; function at activities of daily living (ADL) 91,5±12,1; sport 78,9±20,4; knee-related Quality of life (QOL) 80,0±19,0; total score was 86,6±13,4. Statistically significant association was found between low extremity injuries and sportsrelated PA scores, KOOS subscale of symptoms, pain and QOL scores. Conclusion: Results of the study demonstrated that 1/5 of conscripts were not involved in any kind of sports activity. Almost 50% of conscripts had injuries of lower extremities before military service. KOOS sport-related and QOL subscales’ scores were significantly lower compared to symptoms, pain and ADL related scores. Low extremity injuries were associated with sport-related PA, subjective estimation of knee joint related symptoms, pain and QOL. Key words: Conscripts, physical activity, knee joint pain, injuries of lower extremities, sport, leisure time physical activity.
Genomic imprinting in complex traits
(Tartu : University of Tartu Press, 2018-06) Pervjakova, Natalia