S phase cyclin-CDK specificity in ordering cell cycle phosphorylation
Date
2020
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Abstract
Cell proliferation is an essential process in all organisms. Through a series of growth and
division, the cell passes its genetic material to the next generation of cells. The molecular
machinery that governs timing and execution of these events is called cyclin-dependent
kinase (CDK). CDK is activated by multiple cyclins and phosphorylates hundreds of proteins
associated with the cell cycle. The specificity of phosphorylation is partly dictated by
recognition sequences on proteins called docking motifs that bind specific cyclins. These
docking motifs create a plethora of barcodes that allow CDK to recognize and differentially
phosphorylate many targets. In this work, a novel S-phase specific docking motif NLxxxL
present in CDK inhibitory protein Far1 was mapped and the contribution of cyclin and Cks1
docking on Far1 degradation was analyzed.
In estonian: Rakkude jagunemine on hädavajalik, sest see on aluseks geneetilise materjali ülekandeks
järgmisesse rakkude põlvkonda. Rakutsüklit reguleerivad tsükliinist sõltuvad kinaasid
(CDK), mis fosforüleerivad sadu substraatvalke, mis viivad läbi erinevaid rakutsükli
sündmuseid. CDK substraatide fosforüleerimises mängivad olulist rolli tsükliinid, mis
seonduvad kindlate motiividega substraatvalkudes. Need motiivid võimaldavad CDK
kompleksil erinevatel ajahetkedel rakutsükli jooksul fosforüleerida sadu erinevaid valke.
Käesolevas töös kirjeldati uus S-faasi CDK spetsiifiline tsükliin-substraat seondumismotiiv
NLxxxL ning uuriti erinevate mehhanismide olulisust substraatide fosforüleerimisel.
Description
Keywords
cell cycle, phosphorylation, cyclin-dependent kinase, cyclin, kinase specificity, rakutükkel, fosforüleerimine, tsükliinist sõltuv kinaas, tsükliin, kinaasi spetsiifika