Developing methods for detecting virulence and chemical resistance-related genes of Listeria monocytogenes
Date
2021
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Abstract
Listeria monocytogenes is a pathogen capable of surviving several commonly used chemical
purifying agents, like quaternary ammonium compound-based ones. It provides a challenge for
food processing facilities dealing with the L.monocytogenes infestation. This thesis provides the
theoretical approach to detecting L.monocytogenes pathogenicity and chemical tolerance by
detecting the genes most often implicated in virulence and chemical resistance through the use of
PCR primers. It offers a literature overview of previously discovered information about Listeria
and its pathogenicity and chemical tolerance factors and suggests an experiment plan for PCR
primer design.
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Keywords
Listeria monocytogenes, PCR primer design, L.monocytogenes virulence, L.monocytogenes chemical tolerance