Generation of human papillomavirus type 11 and 18 genomes encoding hemagglutinin epitope-tagged E1 proteins

dc.contributor.advisorPiirsoo, Alla, juhendajaet
dc.contributor.authorLevin, Viktor
dc.contributor.otherTartu Ülikool. Loodus- ja täppisteaduste valdkondet
dc.contributor.otherTartu Ülikool. Molekulaar- ja rakubioloogia instituutet
dc.date.accessioned2018-08-14T08:19:47Z
dc.date.available2018-08-14T08:19:47Z
dc.date.issued2018
dc.description.abstractHuman papillomaviruses (HPVs) are associated with numerous disorders ranging from warts to cancerous diseases. Although vaccination against several types of HPVs is available, a number of HPV-associated cancers remains stable. Therefore, studies on HPV are required for developing novel HPV-targeting drugs. Encoded by HPV E1 helicase is absolutely required for HPV replication. Therefore, it is a good target for developing antiviral drugs. This project aimed at generating HPV11 and HPV18 genomes encoding human influenza hemagglutinin (HA)-tagged E1 proteins with the further goal to test their replication ability in U2OS cell line. The study revealed that HA-tagged E1 proteins maintained the replication of the modified HPV genomes at levels similar to wild-type HPVs (wtHPVs). Thus, this study opens possibilities to investigate HPV11 and HPV18 endogenous E1 proteins using highly specific antibodies against HA epitope and introduces a less expensive and universal method to study E1-associated processes in the HPV life cycle.et
dc.identifier.urihttp://hdl.handle.net/10062/61584
dc.language.isoenget
dc.publisherTartu Ülikoolet
dc.rightsopenAccesset
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Estonia*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/ee/*
dc.subjectE1 proteinet
dc.subjectHA-taget
dc.subjecthuman papillomavirus (HPV)et
dc.subjectreplicationet
dc.subject.otherbakalaureusetöödet
dc.titleGeneration of human papillomavirus type 11 and 18 genomes encoding hemagglutinin epitope-tagged E1 proteinset
dc.typeThesiset

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