Combinatorial Effect of Histone H3 and H4 Mutations with Deletion of YEATS Domain Protein Sas5 in Saccharomyces cerevisiae

Abstract

The single cell eukaryote, Saccharomyces cerevisiae or budding yeast, synthesizes three YEATS domain containing proteins associated with chromatin remodelling and modifying complexes. Previous experiments have shown that deletion of either of the YEATS domain containing proteins, Taf14 or Yaf9, in combination with mutations in the N-terminal tails of histones H3 and H4 is lethal for the cells. In this thesis the aim was to assess the effects of deletion of SAS5, the third YEATS-domain containing protein, in combination with mutations in histones. A decrease in the viability for cells carrying sas5Δ and H3 acetylation and methylation mutations was detected.