Heat shock and chemical-induced stress response of Pseudomonas putida strains

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dc.contributor.advisorJõesaar, Merike, juhendaja
dc.contributor.advisorViggor, Signe, juhendaja
dc.contributor.authorGrigorjeva, Jūlija
dc.contributor.otherTartu Ülikool. Loodus- ja täppisteaduste valdkond
dc.contributor.otherTartu Ülikool. Tehnoloogiainstituut
dc.date.accessioned2024-06-14T10:10:02Z
dc.date.available2024-06-14T10:10:02Z
dc.date.issued2024
dc.description.abstractThe C1 chemicals, such as formate and methanol, are potential feedstocks for sustainable bioproduction. This thesis investigates whether microorganisms that have been genetically modified to assimilate these substances through the reductive glycine pathway face stress. The growth experiments in C1 substrates under heat shock and chemical stress conditions by using stress response reporter systems, pAG032 and pBSibp_Amp, were performed. pBSibp_Amp is a better choice for measuring the heat shock response because it gives much higher ratios than pAG032, and fluorescent bacterial proteins, naturally produced by P. putida and the red fluorescent protein’s fluorescence spectra from plasmid do not overlap. Genetically modified strains can tolerate high concentrations of methanol and formate but do not yield biomass in these growth conditions.
dc.identifier.urihttps://hdl.handle.net/10062/99591
dc.language.isoen
dc.publisherTartu Ülikool
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Estoniaen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/ee/
dc.subjectPseudomonas putida
dc.subjectC1 compounds
dc.subjectHeat shock response
dc.subjectChemical-induced stress response
dc.subjectStress response reporter systems
dc.subject.otherbakalaureusetöödet
dc.titleHeat shock and chemical-induced stress response of Pseudomonas putida strains
dc.title.alternativeKuumashoki ja keemiliste ühendite poolt indutseeritud stressi uurimine Pseudomonas putida tüvedes
dc.typeThesis

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Science and Technology - Bachelor's theses. Kuni 2024