Heat shock and chemical-induced stress response of Pseudomonas putida strains
Date
2024
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Tartu Ülikool
Abstract
The C1 chemicals, such as formate and methanol, are potential feedstocks for sustainable
bioproduction. This thesis investigates whether microorganisms that have been genetically
modified to assimilate these substances through the reductive glycine pathway face stress. The
growth experiments in C1 substrates under heat shock and chemical stress conditions by using
stress response reporter systems, pAG032 and pBSibp_Amp, were performed. pBSibp_Amp
is a better choice for measuring the heat shock response because it gives much higher ratios
than pAG032, and fluorescent bacterial proteins, naturally produced by P. putida and the red
fluorescent protein’s fluorescence spectra from plasmid do not overlap. Genetically modified
strains can tolerate high concentrations of methanol and formate but do not yield biomass in
these growth conditions.
Description
Keywords
Pseudomonas putida, C1 compounds, Heat shock response, Chemical-induced stress response, Stress response reporter systems