Analysis of intercellular network that regulates apicobasal polarity of epithelial cells
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Tartu Ülikool
Abstract
This study examines the apicobasal polarity regulated, by cell-to-cell communication. By
employing conditional knockdown of Scribble, a key apicobasal polarity determinant, in
Drosophila wing imaginal disc, this study aims to identify novel genes that cooperate with
Scribble to regulate cell polarity and tissue homeostasis. Experimental plan is divided into two
parts. First, experimental protocols are tested for establishing screening. Conditional RNAi
method is used. Second, to find out novel genes through systematic screening, small scale
screening is attempted. A combination of conditional RNAi and Dfs stocks in which genes have
been deleted are used. The main objective of the experiment is to identify a strong synergistic
phenotype of neoplasia.
Description
Keywords
Fruit fly, Drosophila melanogaster, wing imaginal disc, Scrib, apicobasal polarity