Co-Expression Analysis of Sigma-1 Receptor and Tetraspanins: Insights into the Biogenesis of Sigma-1 Receptor-Enriched Extracellular Vesicles

Abstract

Sigma-1 receptor (S1R) is a chaperone protein that resides in the endoplasmic reticulum. Recently, S1R has been associated with the generation of extracellular vesicles (EVs). Set of tetraspanins were used as markers for EVs and they were co-express with S1R in mammalian cells using MultiBacMam system. Validation of expression profiles and fluorescent intensities was performed by fluorescence spectroscopy and microscopy. Conditioned cell media was used to isolate EV particles using Tangential Flow Filtration and single-particle analysis with Total Internal Reflection Fluorescence Microscopy was performed to gain insights into the biogenesis of the S1R enriched EVs.