A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

dc.contributor"European Union (EU)" and "Horizon 2020"
dc.contributor.authorRekker, Kadri
dc.contributor.authorAltmäe, Signe
dc.contributor.authorSuhorutshenko, Marina
dc.contributor.authorPeters, Maire
dc.contributor.authorMartinez-Blanch, Juan F.
dc.contributor.authorCodoñer, Francisco M.
dc.contributor.authorVilella, Felipe
dc.contributor.authorSimón, Carlos
dc.contributor.authorSalumets, Andres
dc.contributor.authorVelthut-Meikas, Agne
dc.date.accessioned2019-02-27T08:46:18Z
dc.date.available2019-02-27T08:46:18Z
dc.date.issued2018
dc.description.abstractThe endometrium undergoes extensive changes to prepare for embryo implantation and microRNAs (miRNAs) have been described as playing a significant role in the regulation of endometrial receptivity. However, there is no consensus about the miRNAs involved in mid-secretory endometrial functions. We analysed the complete endometrial miRNome from early secretory (pre-receptive) and mid-secretory (receptive) phases from fertile women and from patients with recurrent implantation failure (RIF) to reveal differentially expressed (DE) miRNAs in the mid-secretory endometrium. Furthermore, we investigated whether the overall changes during early to mid-secretory phase transition and with RIF condition could be reflected in blood miRNA profiles. In total, 116 endometrial and 114 matched blood samples collected from two different population cohorts were subjected to small RNA sequencing. Among fertile women, 91 DE miRNAs were identified in the mid-secretory vs. early secretory endometrium, while no differences were found in the corresponding blood samples. The comparison of mid-secretory phase samples between fertile and infertile women revealed 21 DE miRNAs from the endometrium and one from blood samples. Among discovered novel miRNAs, chr2_4401 was validated and showed up-regulation in the mid-secretory endometrium. Besides novel findings, we confirmed the involvement of miR-30 and miR-200 family members in mid-secretory endometrial functions.et
dc.identifier.urihttps://doi.org/10.3390/genes9120574
dc.identifier.urihttp://hdl.handle.net/10062/63429
dc.language.isoenget
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/692065///WIDENLIFEet
dc.relation.ispartofseriesGenes 2018, 9(12), 574;
dc.rightsinfo:eu-repo/semantics/openAccesset
dc.subjectendometrial receptivityet
dc.subjectinfertilityet
dc.subjectmicroRNAet
dc.subjectrecurrent implantation failureet
dc.subjectsmall RNA-seqet
dc.titleA Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Womenet
dc.typeinfo:eu-repo/semantics/articleet

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