DNA methylation changes in endometrium and correlation with gene expression during the transition from pre-receptive to receptive phase

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dc.contributor"European Union (EU)" and "Horizon 2020"
dc.contributor.authorKukushkina, V.
dc.contributor.authorModhukur, V.
dc.contributor.authorSuhorutšenko, M.
dc.contributor.authorPeters, M.
dc.contributor.authorMägi, R.
dc.contributor.authorRahmioglu, N.
dc.contributor.authorVelthut-Meikas, A.
dc.contributor.authorAltmäe, S.
dc.contributor.authorEsteban, FJ.
dc.contributor.authorVilo, J.
dc.contributor.authorZondervan, K.
dc.contributor.authorSalumets, A.
dc.contributor.authorLaisk-Podar, T.
dc.date.accessioned2019-02-26T13:50:33Z
dc.date.available2019-02-26T13:50:33Z
dc.date.issued2017
dc.description.abstractThe inner uterine lining (endometrium) is a unique tissue going through remarkable changes each menstrual cycle. Endometrium has its characteristic DNA methylation profile, although not much is known about the endometrial methylome changes throughout the menstrual cycle. The impact of methylome changes on gene expression and thereby on the function of the tissue, including establishing receptivity to implanting embryo, is also unclear. Therefore, this study used genome-wide technologies to characterize the methylome and the correlation between DNA methylation and gene expression in endometrial biopsies collected from 17 healthy fertile-aged women from pre-receptive and receptive phase within one menstrual cycle. Our study showed that the overall methylome remains relatively stable during this stage of the menstrual cycle, with small-scale changes affecting 5% of the studied CpG sites (22,272 out of studied 437,022 CpGs, FDR < 0.05). Of differentially methylated CpG sites with the largest absolute changes in methylation level, approximately 30% correlated with gene expression measured by RNA sequencing, with negative correlations being more common in 5′ UTR and positive correlations in the gene ‘Body’ region. According to our results, extracellular matrix organization and immune response are the pathways most affected by methylation changes during the transition from pre-receptive to receptive phase.et
dc.identifier.urihttp://hdl.handle.net/10062/63415
dc.identifier.urihttps://doi.org/10.1038/s41598-017-03682-0
dc.language.isoenget
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/692065///WIDENLIFEet
dc.relation.ispartofseriesSci Rep.;20;7(1):3916
dc.rightsinfo:eu-repo/semantics/openAccesset
dc.subjectTranscriptomicset
dc.subjectGenetics researchet
dc.subjectEpigenomicset
dc.titleDNA methylation changes in endometrium and correlation with gene expression during the transition from pre-receptive to receptive phaseet
dc.typeinfo:eu-repo/semantics/articleet

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