Using genetically encoded fluorescent indicators in Arabidopsis plants
Date
2023
Authors
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Publisher
Tartu Ülikool
Abstract
Genetically-encoded fluorescent indicators (GEFIs) are engineered fluorescent proteins
containing sensory domains that affect excitation and/or emission in response to specific
analytes. In recent years, GEFIs have become a prominent tool in the research of plant signalling
pathways as they allow real-time monitoring molecular events by converting them into readable
and quantifiable signals. This study describes how plant lines with specific mutations and
expressing GEFIs were genotyped, characterized, and employed to detect accumulation of
cytosolic CO2 and H2O2 in response to O3 treatment. The gas exchange experiments with GEFI
lines revealed that ABAleon, an abscisic acid sensor, affected stomatal responses to high CO2
and O3. It was also demonstrated that HPCA1 which recognizes apoplastic H2O2 in plants is
essential for the increase of Ca2+ in the cytosol of plant cells during an exposure to O3.
Description
Keywords
GEFI, Arabidopsis, stomata, calcium, ozone