Optimization of bacterial growth conditions for using fluorescent bioreporters in cell culture infection assays

Abstract

While fluorescent bioreporters are no longer a new thing, they are under constant improvement for application in different fields, and one of them is in medical research. They can be of a great importance for monitoring bacterial infection diseases and give us information about pathogen-drug interaction within the host. In this study, two bioreporters containing the gfp gene (IPTG inducible and constitutively expressing reporter gene) were used in uro-pathogenic E. coli. We tested different bacterial growth conditions and found the optimal one that is not affecting GFP fluorescence and also allow process of bacterial internalization in bladder cells.