Sirvi Autor "Tarassova, Darja" järgi

Tulemuste filtreerimiseks trükkige paar esimest tähte
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  • Pisipilt
    Kirje
    Analysis of intercellular network that regulates apicobasal polarity of epithelial cells
    (Tartu Ülikool, 2022) Tarassova, Darja; Shimmi, Osamu, juhendaja; Tartu Ülikool. Loodus- ja täppisteaduste valdkond; Tartu Ülikool. Molekulaar- ja rakubioloogia instituut
    This study examines the apicobasal polarity regulated, by cell-to-cell communication. By employing conditional knockdown of Scribble, a key apicobasal polarity determinant, in Drosophila wing imaginal disc, this study aims to identify novel genes that cooperate with Scribble to regulate cell polarity and tissue homeostasis. Experimental plan is divided into two parts. First, experimental protocols are tested for establishing screening. Conditional RNAi method is used. Second, to find out novel genes through systematic screening, small scale screening is attempted. A combination of conditional RNAi and Dfs stocks in which genes have been deleted are used. The main objective of the experiment is to identify a strong synergistic phenotype of neoplasia.
  • Pisipilt
    Kirje
    Gene expression profiling of endometrial polyp
    (Tartu Ülikool, 2024) Tarassova, Darja; Saare, Merili, juhendaja; Tõnissoo, Tambet, juhendaja; Pathare, Amruta, juhendaja; Tartu Ülikool. Loodus- ja täppisteaduste valdkond; Tartu Ülikool. Molekulaar- ja rakubioloogia instituut
    Endometrial polyps (EP) are benign growths of endometrial tissue that occur frequently in women, the molecular and genetic causes of which are still unclear. This study compared the transcriptomes of endometrial tissue and EP biopsies to detect changes in gene expression and analyzed cellular diversity using single-cell RNA sequencing. The results of the work showed that the transcriptome profiles of EP and endometrium in tissue biopsies are very similar and only individual differences were detected at the level of gene expression. Single-cell sequencing of the polyp confirmed that the cellular diversity of the EP and endometrium is very similar, but identified three genes (BNC2, CSMD1 and LINC01060) that were differently expressed in the perivascular cell cluster. The importance of these genes in the pathogenesis of EP needs to be confirmed by further studies.