Rakendusliku mõõteteaduse õppekava magistritööd – Master's theses
Permanent URI for this collectionhttps://hdl.handle.net/10062/30310
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Browsing Rakendusliku mõõteteaduse õppekava magistritööd – Master's theses by Issue Date
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Item Experimental realization of the unified pH scale(Tartu Ülikool, 2013) Suu, Agnes; Leito, Ivo, juhendaja; Jalukse, Lauri, juhendajaItem Electron- vibrational coupling in phycobiliproteins of Acaryochloris marina(Tartu Ülikool, 2013) Gryliuk, Galyna; Pieper, Jörg, juhendajaItem ATR-FTIR and multivariate analysis of paints and coating materials(Tartu Ülikool, 2013) Hayes, Philippa Alice; Leito, Ivo, juhendaja; Vahur, Signe, juhendajaItem Cellular Uptake of ARC-Based Inhibitors of Protein Kinases(Tartu Ülikool, 2013) Sinijärv, Hedi; Vaasa, Angela, juhendaja; Uri, Asko, juhendajaItem Energy level structure and protein dynamics in the light-harvesting complex II(Tartu Ülikool, 2013) Vrandecic, Kamarniso; Pieper, Jörg, juhendajaItem Traceability of results and comparison of routine methods in the medical laboratory(Tartu Ülikool, 2013) Ivanov, Agnes; Leito, Ivo, juhendaja; Kallner, Anders, juhendajaItem Validation of the Gas Chromatographic Method for Determination of Volatile Organic Compounds (VOCs) in Industrial Workplace Air(Tartu Ülikool, 2013) Sannik, Sander; Leito, Ivo, juhendajaItem Impact of nuclear data uncertainties on closed nuclear fuel cycle scenarios(Tartu Ülikool, 2013) Atabekjan, Karen; Tkaczyk, Alan Henry, juhendaja; Romanello, Vincenzo, juhendajaItem Building Self-Consistent Scales of Anion Binding by Synthetic Receptor Molecules(Tartu Ülikool, 2013) Haav, Kristjan; Leito, Ivo, juhendajaItem Quantitative analysis of Mitochondrial DNA (mtDNA) copy number in yeast S.cerevisiae cerevisiae strain strain W303 α(Tartu Ülikool, 2013) Hou, Yingjian; Sedman, Juhan, juhendajaItem A method for characterization of vibration testing setups(Tartu Ülikool, 2014) Laizans, Kaspar; Vendt, RihoItem Spectroskopic ellipsometry as a versatile tool to study thin films grown by atomic layer deposition(Tartu Ülikool, 2014) Hoxha, Roland; Kukli, Kaupo; Tamm, AileItem Optimization of ARC-Lum based time-gated photoluminescence method for deternination of affinities of protein kinase inhibitors(Tartu Ülikool, 2014) Wu, Shanshan; Sinijärv, Hedi; Uri, AskoItem Energy level structure and electron - phonon coupling in the light - harvesting complex II(Tartu Ülikool, 2014) Artene, Petrica; Pieper, JörgItem Computational estimation of receptor-anion binding in solution(Tartu Ülikool, 2014) Pung, Astrid; Leito, IvoItem Highly active Ag-MnOx/C catalysts for oxygen electroreduction(Tartu Ülikool, 2015) Shypunov, Illia; Kongi, Nadežda; Tammeveski, KaidoItem Two-flow system for calibration of hygrometers at room temperature(Tartu Ülikool, 2015) Dias, Salgaduge Dinesh Indika; Vilbaste, MartinItem Joint application of an ARC-probe and antibody in homogeneous TR-FRET assay for determination of the concentration of protein kinase Pim2(Tartu Ülikool, 2016) Pagkeu, Sylvestre Tc; Ivan, Taavi; Uri, Asko; Tartu Ülikool. Loodus- ja täppisteaduste valdkondFörster resonance energy transfer (FRET) is a routinely apply photoluminescence technology in high throughput screening in drug discovery. It involves the transfer of energy between two luminescence molecules, the donor and the acceptor in close proximity. In this thesis, we describe the application of the approach exploiting time-resolved measurement of intensity of FRET (TR-FRET) from terbium donor luminophore to fluorescent dye Alexa Fluor 647, for the measurement of the concentration of a specific protein kinase Pim2 in a solution-phase homogenous assay. This method is based on the simultaneous binding of a specific PK to both a luminophore ARC-type probe inhibitor and a fluorescently labelled monoclonal antibody specific to the PK. In case specific IgG labelled with Alexa Fluor 647 acting as acceptor fluorophore is combined in solution with donor luminophore (lanthanide chelate labelled) ARC-probe and the protein kinase of interest, the formation of a triple complex antibody:protein kinase:ARC-probe can be measured by time-resolved measurement of FRET intensity. First, we were able to determine the active concentration of the PK, using a flurescently labelled ARC-type inhibitor of the PK’s active site. Second, we were able to prove the detection of a specific PK by formation of high a FRET signal between a luminophore ARC-type probe PK inhibitor as donor and a fluorescently labelled antibody specific to the PK as acceptor. In addition, formation of the triple complex between ARC-type inhibitor:PK:mAb(Pim2) was confirmed by addition of another ARC-type inhibitor in the well were maximal FRET signal ratio was observed. The determined limit of quantification of the assay was 8 nM, that points to high sensitivity of this homogeneous analytical method. The approach described in this thesis, could be used as a rapid medical diagnostic tool for PK detection and analysis.Item Role of the stringent response in antibiotic tolerance of Escherichia coli(Tartu Ülikool, 2016) Alves Oliveira, Sofia Raquel; Hauryliuk, Vasili; Varik, Vallo; Tartu Ülikool. Loodus- ja täppisteaduste valdkondThe stringent response is a near-universal bacterial adaptation system control mediated by accumulation of two guanine nucleotides ppGpp and pppGpp, collectively known as (p)ppGpp. The response monitors several environmental stress inputs, such as nutrient limitation and heat shock and remodels bacterial physiology in order to overcome the challenges. In Echerichia coli (p)ppGpp levels controlled by two enzymes – RelA and SpoT, the namesakes of RelA SpoT Homologue (RSH) protein family. The stringent response is associated to induction of virulence, antibiotic resistance and was recently suggested to be the driving force behind the formation of so-called persister cells – antibiotictolerant phenotypic variants in antibiotic-sensitive population. Since drug resistance and tolerance constitute a significant public health threat, understanding the connection amongst (p)ppGpp, antibiotic treatment and persistence is of great importance.Item Validation of alpha spectrometric analytical measurement procedure for the determination of Polonium-210 (210Po) in environmental samples(Tartu Ülikool, 2016) Gyakwaa, Francis; Tkaczyk, Alan, juhendaja; Kiisk, Madis, juhendaja; Ozden, Banu, juhendaja; Vaasma, Taavi, juhendaja; Tartu Ülikool. Loodus- ja täppisteaduste valdkondThe thesis aims at finding suitable alpha spectrometry measurement procedure and validating it to be implemented in the laboratory where it can be used for routine analytical procedure for the determination of 210Po in environmental samples. In establishing if the procedure is fit for the intended purpose, the following validation parameters were determined: trueness, accuracy (En-value, z-score), relative bias, and tracer recovery. Also the main sources of uncertainty and their percentage contributions were established.
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