ADA :: Rakendusliku mõõteteaduse õppekava magistritööd – Master's theses sirvimine Kuupäev järgi

Sedimentary dolomite (Es-16, Es-18) and limestone (Es-3, Es-14, Es-17) from Geological Survey of Estonia have been used as reference materials in X-ray fluorescence analysis in Estonian laboratories. Homogeneity of these materials was investigated with laser-ablation inductively coupled plasma mass-spectrometry using MACS-3 and NIST612 certified reference materials for calibration. Natural limestone CAL-S from CRPG was analyzed for overall comparison. Potential application of Es’s as micro-analytical reference materials was evaluated by quantification of uncertainty due to inhomogeneity and estimation of homogeneity index. Es-17 and Es-16 were found most homogenous. More homogenous distribution and higher concentrations of Cs and Rb were found in Es-17, Es-3 and Es-16 than in MACS-3. Inhomogenous distribution of rare earth elements was observed in Es-14, Es-18 and Es-17.

Kirje

Development and validation of gamma spectrometric analysis procedure using a high purity Germanium detector

(Tartu Ülikool, 2016) Sanni, Oluwamayowa Sharon; Tkaczyk, Alan, juhendaja; Goronovski, Andrei, juhendaja; Kiisk, Madis, juhendaja; Tartu Ülikool. Loodus- ja täppisteaduste valdkond

A new detection system, broad energy high purity germanium detector with software (Genie 2000 software) was used to develop a gamma spectrometric analysis procedure. LabSOCS as a tool in Genie was needed to perform a standard-free calibration measurement. Validation of analysis procedure was important to know if the procedure is fit for purpose.

Kirje

Development and validation of UHPLC-MS/MS method for analysis of sedative drugs and their metabolites in blood plasma

(Tartu Ülikool, 2016) Veigure, Ruta; Herodes, Koit; Kipper, Karin; Tartu Ülikool. Loodus- ja täppisteaduste valdkond

In intensive care units, the precise administration of sedative drugs is crucial in order to avoid under- or over sedation – both of which can be very harmful. It is especially important in case of paediatric patients. A highly sensitive simultaneous UHPLC-MS/MS method was developed for the quantification of morphine, morphine-3-β-glucuronide, morphine-6-β-glucuronide, clonidine, midazolam and 1’-hydroximidazolam in human plasma samples. The lowest limit of quantification for all analytes was 50 pg/mL using only 100 μL of blood plasma. Analytes were separated chromatographically using C18 column with weak ion-pairing additive 1,1,1,3,3,3-hexafluoro-2-propanol with pH = 9 (adjusted using ammonium hydroxide) and methanol. Method was fully validated and method was using matrix matched calibration in the range of 0.05-250 ng/mL for all analytes. Within-day accuracy for all analytes remained 87-113 %, but within-day precision remained within 3-11 % for all analytes at all concentration levels over the calibration range. The method will be applied for real patients’ samples in the EU FP7 project CloSed – "Clonidine for Sedation of Paediatric Patients in the Intensive Care Unit”.

Sirvi

Sirvi Rakendusliku mõõteteaduse õppekava magistritööd – Master's theses Kuupäev järgi