Analysis of intercellular network that regulates apicobasal polarity of epithelial cells

Abstract

This study examines the apicobasal polarity regulated, by cell-to-cell communication. By employing conditional knockdown of Scribble, a key apicobasal polarity determinant, in Drosophila wing imaginal disc, this study aims to identify novel genes that cooperate with Scribble to regulate cell polarity and tissue homeostasis. Experimental plan is divided into two parts. First, experimental protocols are tested for establishing screening. Conditional RNAi method is used. Second, to find out novel genes through systematic screening, small scale screening is attempted. A combination of conditional RNAi and Dfs stocks in which genes have been deleted are used. The main objective of the experiment is to identify a strong synergistic phenotype of neoplasia.