Oreksiiniretseptori geeni HCRTR1 RS2271933 polümorfismi seos aeroobse võimekusega 9- ja 15-aastastel lastel

Abstract

Aim: To determine the effect of the orexin receptor gene HCRTR1 rs2271933 polymorphism on aerobic capacity in a sample of 9-and 15-year-old children from the Estonian Children Personality, Behaviour and Health Study (ECPBHS). Additionally, the study examined whether the genotype’s effect depends on the subjects’ age, sex, and training load. Methods: The study used data from the first wave of the ECPBHS. In total, data from 1168 participants were used in the analyses. Participants were divided into two age cohorts: 9- and 15-year-olds. Aerobic capacity was measured via incremental bicycle ergometer exercise test. Genotyping was performed using real-time polymerase chain reaction. Information about physical activity levels was conducted through self-reported questionnaires and the resulting data were used as a covariate in the statistical analysis. Results: Carriers of the AG genotype of the HCRTR1 rs2271933 polymorphism showed significantly higher aerobic capacity compared to GG genotype carriers. Depending on the subgroup, AG carriers had a mean aerobic capacity 0.10−0.18 W/kg higher than GG carriers. The effect of the genotype was sex- and age-specific: in the main effect analysis, the effect was significant only in the 9-year-old cohort and in the pooled analysis of girls (ages 9 and 15). After adjusting for training load as a covariate, the HCRTR1 genotype effect became statistically significant for the total sample, 9-year-old boys and 15-year-old girls. In subgroups where the effect was already significant in the main effect analysis, the association strengthened after including training load as a covariate. Conclusions: The AG genotype of the HCRTR1 rs2271933 polymorphism has a positive effect on aerobic capacity. However, the effect is sex- and age-specific, manifesting in boys before puberty and in girls during adolescence. The influence of the HCRTR1 rs2271933 polymorphism on aerobic capacity appears to be biologically independent, regardless of the children’s training load. Keywords: HCRTR1, rs2271933, aerobic capacity, orexin receptor